Presentation Authors: Won Tae Kim*, Ho Won Kang, Sung Pil Seo, Cheonju, Korea, Republic of, In-Chang Cho, Seoul, Korea, Republic of, Yong-June Kim, Seok-Joong Yun, Sang-Cheol Lee, Wun-Jae Kim, Cheonju, Korea, Republic of
Introduction: on-muscle-invasive BC (NMIBC) is a malignant disease characterized by high recurrence and progression rates, which constitutes significant challenge. At present, urinary cell-free DNA (ucf DNA) is widely considered as a potential biomarker for the noninvasive diagnosis of bladder cancer (BC). In this study, we aimed to identify genetic signatures associated with NMIBC in ucf DNA for the progression monitoring.
Methods: A cohort of 103 patients presenting NMIBC (pTa-pT1) were enrolled. The expression levels of ucf DNA IQGAP3/BMP4 and IQGAP3/FAM107A were then detected by quantitative real-time PCR analysis, and the results were correlated with clinical data using Kaplan-Meier curves and Cox regression analyses. Progression was defined as development of muscle invasion or metastasis.
Results: Overall, 55 (53.4%) patients recurred and 29 (28.2%) patients progressed during the median follow-up of 43.2 (21.5-94.5) months. The High expression levels of ucf DNA IQGAP3/BMP4 and IQGAP3/FAM107A in NMIBC were associated with worse clinical outcome. Kaplan-Meier analysis showed that NMIBC patients with high IQGAP3/BMP4 lever had worse recurrence-free survival (RFS), progression-free survival (PFS) (P=0.002 and < 0.001, respectively) and those with low IQGAP3/FAM107A level had better PFS (P=0.005). Multivariate Cox regression analysis revealed that IQGAP3/BMP4 was independently associated with RFS, PFS of NMIBC (P=0.005 and 0.001 respectively), and IQGAP3/FAM107A independently related to PFS of NMIBC (P=0.048).
Conclusions: Ucf DNA IQGAP3/BMP4 and IQGAP3/FAM107A may have considerable clinical value in prognosis prediction of BC.