Presentation Authors: Zhang Guoxi, Qiu Kaiyang, Zou xiaofeng*, Liu Quanliang, Xie Tianpeng, He zhihua, Ganzhou, China, People's Republic of
Introduction: To explore the correlation and possible mechanism between mir-193a-3p and adrenal aldosterone tumor.
Methods: The total RNA of 15 patients with adrenal aldosterone tumor were collected after operation from aldosterone tumor and adjacent normal adrenal gland. The expression of mir-140-3p, mir-193a-3p, mir-22-3p and CYP11B2 mRNA were detected by quantitative reverse transcription PCR (qrt-pcr). Cultured adrenocortical cell line H295R and transfected with mir-193a-3p. Cell proliferation was detected by colorimetry and apoptosis and cell cycle progression were analyzed by flow cytometry. The levels of aldosterone in the supernatant were detected by ELISA.
Results: By detecting three miRNAs, miR-193a-3p expression was significantly decreased and CYP11B2 mRNA expression was significantly increased in APA compared with adjacent normal adrenal tissue. The miR-193a-3p mimic was transfected into the human adrenocortical cell line H295R, and it was found that increased expression of miR-193a-3p inhibited cell proliferation and aldosterone secretion, induced G1 arrest and promoted apoptosis of H295R cells. In addition, by luciferase gene assay, overexpression of miR-193a-3p in H295R cells significantly reduced the luciferase activity of the wild-type CYP11B2 3'-UTR construct and found that it can pass miR-193a- Reversal of mutation of the 3p binding site. In addition, miR-193a-3p overexpression down-regulated the expression of CYP11B2 mRNA and protein. Finally, overexpressed CYP11B2 attenuated the effect of miR-193a-3p on H295R cells.
Conclusions: Our study suggests that miR-193a-3p may be involved in down-regulating the expression of CYP11B2 and inhibiting APA growth. This also explains that down-regulation of miR-193a-3p promotes cell growth and inhibits apoptosis during APA formation.