CSAS Oral Student Competition: MS Session
This in vitro study assessed the effects of three pine-based engineered biocarbons (CP002, CP016, CP023) that differed in particle size (< 0.5, 0.5-2.0, < 2.0 mm) at two inclusion levels (0.25 mg/ml and 0.5 mg/ml) on total gas and CH4 production as well as rumen fermentation parameters when added to a barley silage-based total mixed ration diet. Biocarbon products differed in bulk density, surface area, pore volume, and pH but had similar chemical compositions. The control consisted of the barley-silage diet only. In each jar, 0.5g DM of the total mixed ration was supplemented with the above biocarbon treatments. These jars were incubated for 48 hr at 39 °C. Gas samples were collected at 3, 6, 9, 12, 24, 36 and 48 hr. Data were analysed by using PROC MIXED in SAS in a randomized complete block design with treatment, rate, and particle size as fixed effects and run and replicate as random effects. Methane production was not affected by treatment (p=0.37), inclusion rate (p=0.57) or particle size (p=0.39). Gas production linearly increased (p< 0.01) with biocarbon. Digested DM was not affected (p=0.23) by biocarbon addition. Additionally, treatment, rate and particle size had no effect (p >0.05) on pH, VFA and ammonia N concentrations. In conclusion, the amount of biocarbon differing particle sizes at different rates did not affect CH4 emissions; but did increase gas production.