Award: Presidential Poster Award
Lamyaa Soliman, MSc1, Rania Zayed, MD1, Dalia Omran, MD1, Fadwa Said, MD1, Samar Darweesh1, Doaa Ghaith, MD1, Rasha Eletreby, MD1, Mahmoud Barakat1, Mahmoud Bendary2, Doaa Zaky3, Eman Amer4, Iman Elmahgoub, MD1
1Cairo University, Cairo, Al Qahirah, Egypt; 2Port Said University, Bur Sa'id, Bur Sa'id, Egypt; 3Ain Shams University, Cairo, Al Qahirah, Egypt; 4Ahram Canadian University, Al Jizah, Egypt
Introduction: Portal hypertension (PH) and esophageal varices complicating HCV-related chronic liver disease (CLD) are the most devastating sequelae. Angiogenesis is the hallmark of their pathogenesis. Apelin is one of the recently identified angiogenic and fibrogenic peptides.
We studied apelin gene expression, apelin (rs3761581) SNP and serum apelin level in chronic HCV patients, and their association with hepatic fibrosis and esophageal varices.
Methods: 112 patients with HCV related CLD (40 having liver cirrhosis (LC)/low grade varices, 33 with LC/high grade varices and 39 with fibrotic non-cirrhotic liver/no varices), and 80 healthy control subjects were included. Real time PCR was used for apelin gene expression assay and apelin rs3761581 SNP analysis in peripheral blood samples. Serum apelin level was measured by ELISA.
Results: Apelin gene expression was undetectable in studied samples. SNP analysis revealed higher frequency of the C-(mutant)-allele among patients compared to controls (p =0.012, odds ratio 3.67). Serum Apelin level was significantly higher in patients with hepatic cirrhosis/ varices (median 31.6 ng/L) compared to non-cirrhotic patients/ no varices (median 2.9 ng/L) (p< 0.001). Serum apelin level at cut off value 16.55 ng/L predicted the presence of varices with AUROC 0.786. Positive correlation was found between serum apelin level and grade of liver fibrosis (r=0.346, p< 0.001) and PH (r=0.438, p< 0.001).
Discussion: Apelin rs3761581 C-allele may be associated with progression of HCV-related CLD and varices formation, and can be considered as a potential therapeutic target to control fibrosis progression. Serum apelin level provided accurate prediction of the presence of esophageal varices.
Citation: Lamyaa Soliman, MSc; Rania Zayed, MD; Dalia Omran, MD; Fadwa Said, MD; Samar Darweesh; Doaa Ghaith, MD; Rasha Eletreby, MD; Mahmoud Barakat; Mahmoud Bendary; Doaa Zaky; Eman Amer; Iman Elmahgoub, MD. P0398 - APELIN ASSOCIATION WITH HEPATIC FIBROSIS AND ESOPHAGEAL VARICES IN CHRONIC HCV PATIENTS. Program No. P0398. ACG 2019 Annual Scientific Meeting Abstracts. San Antonio, Texas: American College of Gastroenterology.