Basic Science: Oncology
Moderated Poster Session
Introduction & Objective : To determine the role of SHC SH2-domain binding protein 1 (SHCBP1) in prostate cancer (PCa) progression using human PCa tissues and cell lines.
Methods : We collected 269 PCa tissues and 80 benign prostatic hyperplasia (BPH) tissues as controls between January 2007 and January 2010 at our center. Clinicopathological characteristics were then analyzed using tissue microarray and immunohistochemistry, and Cox regression analyses were applied to explore independent factors correlated with SHCBP1 expression; biochemical PCa recurrences were also evaluated. Then, we compared SHCBP1 expression in PC-3, DU 145 and LNCaP cells by qRT-PCR, and silenced SHCBP1 in PC-3 cells using siRNA. Cell proliferation and tumorigenicity were then studied by Celigo image cytometry, FACS, MTT assay, transwell assay, wound-healing assay and western blotting.
Results : Our immunohistochemical analysis showed that SHCBP1 was significantly upregulated in PCa tissues compared with BPH tissues and was correlated with poor prognosis. SHCBP1 silencing inhibited the proliferation and tumorigenicity of PC-3 cells in vitro.
Conclusions : Our findings show that SHCBP1 is a novel target gene for PCa and demonstrate that SHCBP1 upregulation promotes invasiveness in vitro and is correlated with poor prognosis in patients.
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