Clinical Oncology: Outcomes & Complications

Moderated Poster Session

MP18-20 - Retrospective analysis of the concordance of 20 immunohistochemical tissue markers in metastasectomy lesions in patients with metastatic renal cell carcinoma

Saturday, September 22
10:00 AM - 12:00 PM
Location: Room 253

Introduction & Objective :

 This study was aimed at characterizing the different expressions of 20 tissue markers in multiple metastatic lesions and organs in patients with metastic renal cellc acrcinoma (mRCC).


Methods : A total of 66 patients with mRCC, harboring 179 metastasectomy tissue lesions (MTLs), were enrolled with their medical records. Immunohistochemistry analysis was performed for tissue microarrays using 20 tissue markers: BAP1; CD31; CD 34; HIF-1α and -2α; Ki-67; pS6; PBRM1; PDGFRa and b; PD-L1; PSMA; PTEN; α-SMA; TGase2; VEGFR-1, -2, and -3; VHL loss; and CA9. The cases were identified pathologically using the semi-quantitative H-score (0–300). The concordance rate was calculated as the number of patients with concordant intensity score out of the total number of patients in that comparison


Results :

A total of 66 patients were divided into those with the same organs and those with different organs. Forty-two patients (44 cases) with 96 MTLs and 39 with 83 MTLs were used to examine the concordance of the markers within the same and different organs, respectively. Among 179 MTLs, 84 from the kidneys, 43 from the lungs, 25 from the bone, 7 from the brain, 5 from the lymph nodes (LNs), and 15 from other organs were stained with tissue markers. Within the same organs, the BAP1, PSMA, VEGFR3, PDGFRa (>0.9 concordance rate), PS6, HIF-2 (>0.8 concordance rate), PD-L1, and HIF-1 (>0.7 concordance rate) exhibited highly concordant expressions, even though they originated from different MTLs. As for the expression of different metastatic organs in the same patient, BAP1, PSMA, HIF-1, VEGFR3 (>0.9 concordance rate), PDGFRa, SMA (>0.8 concordance rate), PS6, PD-L1, and PTEN (>0.7 concordance rate) showed consistent results, despite being obtained from different organs. For 26 patients who exhibited recurrence within the same organ, the tissue samples were re-stained for the markers, and MTLs were analyzed to examine the concordance of the recurrent site within the same organ. BAP1, PSMA (>0.9 concordance rate), PS6, VEGFR3, PDGFRa (>0.8 concordance rate), PTEN, and HIF-2 (>0.7 concordance rate) showed concordance. However, there was a limitation in that the distribution of some of the high concordant markers had shifted to one side.


Conclusions :

The high-concordance tissue markers, including BAP1, VEGFR3, PDGFRa, and PSMA (PS6: concordance rate >0.7), showed a similar expression of markers regardless of metastatic organs, metastatic sites, or recurrence, whereas other markers were influenced by other factors.

Sung Han Kim

Assistant professor
Department of Urology, Prostate Cancer Center, National CanceNational CAncer Centerr Center, Goyang, Korea

Sung Han Kim has completed his MD at the age of 30 years from Seoul National University of Medicine, Seoul, Rep. of Korea and postdoctoral study from National Cancer Center, Goyang, Korea and Seoul National University of Medicine, Seoul , Korea.
He is now the Clinical Staff and Associate Researcher under the director of Jinsoo Chung (from Prostate Cancer Center, National Cancer Center, Goyang, Korea).
He publishes 15 articles per year and has published more than 80 papers in reputed cancer journals.

mobile H.P: +82-10-4248-7762,
Office Tel: +82-31-920-1706
e-mail : 12112@ncc.re.kr

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    Weon Seo Park

    Department of Pathoogy, Prostate Cancer Center, National Cancer Center, Goyang, Korea

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      Eun Young Park

      Biometrics branch, Research institute and National Cancer Center, Goyang, Korea

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        Jungnam Joo

        Biometrics branch, Research institute and National Cancer Center, Goyang, Korea

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          Jinsoo Chung

          Department of Urology, Prostate Cancer Center, National Cancer Center, Goyang, Korea

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