Category: Basic Science: Reconstruction

MP3-10 - Cyclic hydrostatic pressure promotes the expression of uroplakins in human urothelial cells by activation of ERK1/2 signaling pathway

Thu, Sep 20
4:00 PM - 6:00 PM

Introduction & Objective :

To investigate the effect of cyclic hydrostatic pressure on the expression of uroplakins and the role of extracellular regulated protein kinases 1/2 (ERK1/2) in the hydrostatic pressure-induced uroplakins expression of human urothelial cells (HUCs).


Methods :

 Human UCs were seeded into a cell culture flask and subjected to cyclic hydrodynamic pressures for 24 h. Pressure parameters were set as follows: static, 100 cm H2O, 200 cm H2O and 300 cm H2O pressure. Real-time polymerase chain reaction (RT-PCR) and western blot were used to detect the expression of uroplakins. The role of the ERK1/2 was investigated using ERK1/2 inhibitor.


Results :

We used RT-PCR to detect the genes transcription of UPIa, UPIb, UPII and UPIII. Our results showed that hydrostatic pressure promoted the transcription of uroplakins, especially in 200 cmH2O group (p 0.05)  (Fig. 1B). Results showed that cyclic hydrostatic pressure significantly promoted the protein expression of ERK1/2 and p-ERK1/2 (Thr202/Tyr204) (p < 0.05) (Fig. 2A and 2B). To define the role of ERK1/2 in pressure-induced uroplakins expression, human UCs were pretreated with the inhibitor of ERK1/2. As demonstrated in Fig. 3, ERK1/2 inhibitor significantly inhibited the protein expression of UPIa and UPII (P < 0.05).


Conclusions :

In summary, we confirmed the role of cyclic hydrostatic pressure in regulating the expression of uroplakins. More importantly, our experiments have also demonstrated that ERK1/2 is an important signal molecule in regulating this expression. These results provide potential intervention targets for overexpression of uroplakins in bladder outlet obstruction and provide mechanical parameters for generating mature seed cells for tissue-engineered bladder.

Yuntian Chen

Student
Department of Urology, West China Hospital, Sichuan University
Chengdu, Sichuan, China (People's Republic)

Chen Yuntian, student in the urology department in sichuan university, western china hospital.

Xiaoshuai Gao

student
Department of Urology, Institute of Urology (Laboratory of Reconstructive Urology), West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.
Chengdu, Sichuan, China (People's Republic)

Kunjie Wang

Chengdu, Sichuan, China (People's Republic)

Hong Li

Chengdu, Sichuan, China (People's Republic)

Qing He

Department of Urology, West China Hospital, Sichuan University, No. 37 Guo Xue Xiang, Chengdu, Sichuan, 610041, P.R. China.
Department of Urology, Institute of Urology (Laboratory of Reconstructive Urology), West China Hospital, Sichuan University
Chengdu, Sichuan, China (People's Republic)

Mr. Qing He has been studying Clinical Medicine at West China School of Medicine of Sichuan University since 2016. He is now a doctoral degree candidate, majoring in Urology.