Category: Basic Science: Oncology
Introduction & Objective :
We evaluated the expression levels of let-7c, a well-known oncosuppressor miRNA, in urine and neoplastic tissue in patients with high grade Ta/T1 stage Urothelial Carcinoma, matched with healthy controls.
Thirty-eight urine and tissue samples were collected from TURB of patients with high risk NMIBC. Ten urine and 5 bladder tissue samples
were collected as healthy controls. Urine samples were centrifuged at 4.500 rpm for 30 minutes at 4Â°C, next at 8.900 rpm for 5 minutes at 4Â°C. The supernatant was separated from the cellular pellet, collected in 2 mL criovials and frozen at -80Â°C. Different portions of paired tumoral tissue samples were collected in 2 mL criovials and frozen in liquid nitrogen. RNA was isolated from urine and tissue samples. Analysis of miRNA expression levels was performed by qRTâ€“PCR and mean levels compared between bladder cancer and healthy control cohorts with Student t test. The impact of let-7c expression on recurrence free and progression free survival of bladder cancer patients was assessed with Kaplan Meier method.
Clinical features of the cohort are reported into Table 1. When matched with 10 healthy controls, let-7c was overexpressed in urine samples (p=0.039); conversely, tissue sample analysis showed lower let-7c miRNA levels in bladder cancer patients, compared to five healthy controls (p33.2% and 83.2 %, respectively. At Kaplan Meier analysis, urinary Let-7c hyperexpression did not predict disease recurrence (log rank p=0.741) but was associated to poorer progression free survival (log rank p=0.007). Fig. 1
Let-7c expression in both urine and tissue samples provides a significant discrimination between patients with urothelial NMIBC versus healthy controls. A preliminary analysis of urine samples in NMIBC cohort highlighted a potential role of let-7c urine hyperexpression as predictor of disease progression to muscle invasive disease.
Mariaconsiglia Ferriero– MD, PhD, "Regina Elena" National Cancer Institute, Dept. of Urology, Rome, Lazio, Italy
Manuela Spagnuolo– Rome, Lazio, Italy
Manuela Costantini– "Regina Elena” National Cancer Institute, Dept. of Urology, Rome, Lazio, Italy
Marco Varmi– Rome, Lazio, Italy
Vincenzo Pompeo– Rome, Lazio, Italy
Giulia Piaggio– Rome, Lazio, Italy
Michele Gallucci– Roma, Lazio, Italy
Giuseppe Simone– Consultant, “Regina Elena” National Cancer Institute, Dept. of Urology, Roma, Lazio, Italy
Maria Giulia Rizzo– Rome, Lazio, Italy
"Regina Elena" National Cancer Institute, Dept. of Urology
Rome, Lazio, Italy
"Regina Elena” National Cancer Institute, Dept. of Urology
Rome, Lazio, Italy
“Regina Elena” National Cancer Institute, Dept. of Urology
Roma, Lazio, Italy