Category: Basic Science: Oncology

MP1-7 - Urine let- 7c is able to discriminate high grade non-Muscle Invasive Bladder Cancer patients from healthy subjects

Thu, Sep 20
4:00 PM - 6:00 PM

Introduction & Objective :

We evaluated the expression levels of let-7c, a well-known oncosuppressor miRNA, in urine and neoplastic tissue in patients with high grade Ta/T1 stage Urothelial Carcinoma, matched with healthy controls.


Methods :

Thirty-eight urine and tissue samples were collected from TURB of patients with high risk NMIBC. Ten urine and 5 bladder tissue samples
were collected as healthy controls. Urine samples were centrifuged at 4.500 rpm for 30 minutes at 4°C, next at 8.900 rpm for 5 minutes at 4°C. The supernatant was separated from the cellular pellet, collected in 2 mL criovials and frozen at -80°C. Different portions of paired tumoral tissue samples were collected in 2 mL criovials and frozen in liquid nitrogen. RNA was isolated from urine and tissue samples. Analysis of miRNA expression levels was performed by qRT–PCR and mean levels compared between bladder cancer and healthy control cohorts with Student t test. The impact of let-7c expression on recurrence free and progression free survival of bladder cancer patients was assessed with Kaplan Meier method.


Results :

Clinical features of the cohort are reported into Table 1. When matched with 10 healthy controls, let-7c was overexpressed in urine samples (p=0.039); conversely, tissue sample analysis showed lower let-7c miRNA levels in bladder cancer patients, compared to five healthy controls (p33.2% and 83.2 %, respectively. At Kaplan Meier analysis, urinary Let-7c hyperexpression did not predict disease recurrence (log rank p=0.741) but was associated to poorer progression free survival (log rank p=0.007). Fig. 1


Conclusions :

Let-7c expression in both urine and tissue samples provides a significant discrimination between patients with urothelial NMIBC versus healthy controls. A preliminary analysis of urine samples in NMIBC cohort highlighted a potential role of let-7c urine hyperexpression as predictor of disease progression to muscle invasive disease.

Mariaconsiglia Ferriero

MD, PhD
"Regina Elena" National Cancer Institute, Dept. of Urology
Rome, Lazio, Italy

Manuela Spagnuolo

Rome, Lazio, Italy

Manuela Costantini

"Regina Elena” National Cancer Institute, Dept. of Urology
Rome, Lazio, Italy

Marco Varmi

Rome, Lazio, Italy

Vincenzo Pompeo

Rome, Lazio, Italy

Giulia Piaggio

Rome, Lazio, Italy

Michele Gallucci

Roma, Lazio, Italy

Giuseppe Simone

Consultant
“Regina Elena” National Cancer Institute, Dept. of Urology
Roma, Lazio, Italy

Maria Giulia Rizzo

Rome, Lazio, Italy