Category: New Technology: Miscellaneous

MP22-2 - A novel method for in vivo isolation of urothelial cancer cells in urine using CellCollector - a functionalized and structured medical wire under cystoscopy

Sat, Sep 22
2:00 PM - 4:00 PM

Introduction & Objective : The purpose of this study is to evaluate performance and practical applicability of a novel method for in vivo isolation of urothelial cancer cells (UCC) in urine using CellCollector, a functionalized and structured medical wire under cystoscopy.


Methods : The CellCollector is comprised of stainless steel wire widely used in medicine. Antibodies to the epithelial cell surface antigen EpCAM are attached to a polycarboxylate/hydrogel which is coated on a gold-plated layer. The CellCollector captures target cells expressing EpCAM antigen on cell surface. We inserted CellCollector into bladder through the scope sheath until the functional domain of the device was exposed to urine and kept it staying in the bladder for at least 10 minutes while conducting cystoscopy. UCC in urine captured by the CellCollector was identified by immunocytochemical staining for EpCAM, CD45, Hoechst and P53/CK20. Intensity of the immunocytochemical staining of captured cells was evaluated using an Axio Imager A1m microscope equipped with an AxioCam digital camera system and the AxioVision 4.6 software. PBMC and MCF7 cell lines were used as negative and positive control for each case.


Results : We applied this novel method to 10 patients with bladder cancer, 10 patients with benign prostatic hyperplasia (BPH) and 10 patients with interstitial cystitis (IC). We defined EpCAM+/CD45-/Hoechst+ and P53/CK20+ cells as UCC. Among bladder cancer patients, detection were all performed before transurethral resection of bladder tumor (TURBT) procedure. The number of all captured cells per patient with bladder cancer is 86±21. UCC were found in each of them, the number of which is 38±12. The percentage of UCC in all captured cells varied from 18.1% to 42%. Among patients with BPH and IC, the number of all captured cells per patient are 24±16 and 18±10. None UCC were found in these two groups. Within all 30 cases, no device related complications were observed.


Conclusions : The sensitivity of standard urine cytology for detecting urothelial cell carcinoma is suboptimal, due to its inability to process entire samples and presence of background cells. Our present findings indicate that such an in vivo isolation platform is a promising approach to capture sufficient amounts of UCC while conducting cystoscopy. Comparing with finding a fish in a bottle of water, we use a fishing rod to attract fish in the ocean.

Liang Dong

Resident
Renji Hospital, Shanghai Jiao Tong University School of Medicine
Shanghai, Shanghai, China (People's Republic)

Ming Cao

Shanghai, Shanghai, China (People's Republic)

Shengqiang Xia

Shanghai, Shanghai, China (People's Republic)

Hai Zhong

Shanghai, Shanghai, China (People's Republic)

Wenhao Shen

Shanghai, Shanghai, China (People's Republic)

Haige Chen

Shanghai, Shanghai, China (People's Republic)

Wei Xue

Shanghai, Shanghai, China (People's Republic)

Yiran Huang

Shanghai, Shanghai, China (People's Republic)