Category: Basic Science: Oncology
Introduction & Objective : Warm renal ischemia (WRI) is commonly performed in partial nephrectomy, during small renal masses treatment. However, WRI is associated with remaining parenchyma damage. Thus, several drugs have been studied for using as renal protectors during partial nephrectomy. One of those is resveratrol, a bioflavonoid used as antioxidant in several situations. Nevertheless there are no quantitative results of the treatment with resveratrol for WRI. The objective of this study are to quantitatively evaluate renal cortex morphology after warm ischemia and the possibleprotective effect of resveratrol.
We used 38 male Wistar rats at aging 9 weeks divided into 4 groups: Sham (S) – 60 minutes laparotomy; Sham Resveratrol (SR) – sham group treated with resveratrol; Ischemia (I) – group submitted to renal ischemia; Ischemia Resveratrol (IR) – group submitted to renal ischemia and treated with resveratrol. The SR and IR groups received 30mg/kg of resveratrol intraperitoneally 60 minutes before the surgical procedure.. The I and IR groups had their left renal vessels clamped for 60 minutes, while S and SR had renal pedicle dissected but not clamped. The animals were submitted to euthanasia 4 weeks after the procedure, and had their kidneys collected for histomorphometric analysis. The renal volume, the cortex-non-cortex areas ratio (C/NC), glomerular volume density (Vv[Glom]), weighted mean glomerular volume (VWGV) and number of glomeruli per kidney (Nv[Glom]) were evaluated. The results were compared by one-way ANOVA with Bonferroni post-test and considered significant when the value of p<0.05.
Results : The serum urea in group I was increased in relation to the S group by 9.6%, and no significant differences were observed in IR group when compared to groups S and SR (p=0.01). The C/NC of the group IR did not show significant differences in comparison with non-ischemic groups (S and SR) (p<0.01). The Vv[Glom] of the group IR was higher in 32.8% when compared to the group I and did not present significant differences when compared to groups S and SR (p<0.01). The Nv[Glom] of group IR presented itself increased in 26.2% when compared to the group I and did not show significant differences when compared to groups S and SR (p=0.01).RV (p=0.13) and VWGV(p=0.15) had no significant difference among groups.
Conclusions : Warm renal ischemia induces important renal morphological and functional prejudice. Resveratrol prevented these alterations and should be further tested for clinical use during partial nephrectomies.
Francisco Sampaio– Full Professor and Head, State University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil
Gabriela Gonçalves– Rio de Janeiro, Rio de Janeiro, Brazil
Bianca Gregório– State University of Rio de Janeiro, Rio De Janeiro, Rio de Janeiro, Brazil
Marco Pereira-Sampaio– Urogenital Research Unit, Rio De Janeiro, Rio de Janeiro, Brazil
Diogo De Souza– Urogenital Research Unit, Rio De Janeiro, Rio de Janeiro, Brazil
Full Professor and Head
State University of Rio de Janeiro
Rio de Janeiro, Rio de Janeiro, Brazil
Anatomist and Urologist
Full-Professor, Biomedical Center, State University of Rio de Janeiro
Director of the Urogenital Research Unit
Senior Investigator (I-A), National Council of Research (CNPq), Ministry of Science and Technology
Scientist of State of Rio de Janeiro (Foundation for Research Support - RJ)
General Coordinator, All National Programs of Postgraduation in Surgery and Surgical Specialties - Government Postgraduation Agency (CAPES), Ministry of Education, 2005 - 2010
Full-Member, National Academy of Medicine, Brazil, Past President 2015-2017
State University of Rio de Janeiro
Rio De Janeiro, Rio de Janeiro, Brazil