Category: Basic Science: Oncology
Introduction & Objective : Injury caused by ischemia/reperfusion in the kidney remains an important issue regarding nephron sparing surgery. Thus, drugs that protect the kidney during these procedures are desired. Quercetin is a natural drug whose protective effects on the kidney and other organs have been evaluated under different conditions, but there are still no quantitative results of the use of this flavonoid after warm renal ischemia. The objective of this study is to quantitatively evaluate the renal cortex histomorphology after warm ischemia and the possible protective effect of quercetin.
Thirty eight adult male rats were divided into 4 groups: Sham (S) – 60 minutes laparotomy; Sham Quercetin (SQ) – sham group treated with quercetin; Ischemia (I) – group submitted to renal ischemia; Ischemia Quercetin (IQ) – group submitted to renal ischemia and treated with quercetin. The SQ and IQ groups received 50 mg/kg of quercetin by gavage from 3 days before and to 3 days after surgery, and one intraperitoneal administration before the surgical procedure. The I and IQ groups had their left renal vessels clamped for 60 minutes, while S and SQ had renal pedicle dissected but not clamped. The animals were submitted to euthanasia 4 weeks after the procedure, and had their kidneys collected for histomorphometric analysis. The renal volume, the cortex-non-cortex areas ratio (C/NC), glomerular volume density (Vv[Glom]), weighted mean glomerular volume (VWGV) and number of glomeruli per kidney (Nv[Glom]) were evaluated. The results were compared by one-way ANOVA with Bonferroni post-test and considered significant when the value of p
Results : Group I showed a 9.6% increased serum urea in comparison to group S, while no difference was observed in group IQ (p<0.01).The C/NC, Vv[Glom] and N[Glom] of group I was reduced in comparison to other groups, while quercetin increased the results of this parameter. Renal volume and VWGV did not present significant differences among groups.
Conclusions : Arteriovenous warm renal ischemia promotes considerable damage to the kidney and previous administration of quercetin has protective effects to the renal cortex, suggesting that this drug can be used preventively in partial nephrectomy.
Francisco Sampaio– Full Professor and Head, State University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil
Gabriela Gonçalves– Rio de Janeiro, Rio de Janeiro, Brazil
Bianca Gregório– State University of Rio de Janeiro, Rio De Janeiro, Rio de Janeiro, Brazil
Marco Pereira-Sampaio– Urogenital Research Unit, Rio De Janeiro, Rio de Janeiro, Brazil
Diogo De Souza– Urogenital Research Unit, Rio De Janeiro, Rio de Janeiro, Brazil
Full Professor and Head
State University of Rio de Janeiro
Rio de Janeiro, Rio de Janeiro, Brazil
Anatomist and Urologist
Full-Professor, Biomedical Center, State University of Rio de Janeiro
Director of the Urogenital Research Unit
Senior Investigator (I-A), National Council of Research (CNPq), Ministry of Science and Technology
Scientist of State of Rio de Janeiro (Foundation for Research Support - RJ)
General Coordinator, All National Programs of Postgraduation in Surgery and Surgical Specialties - Government Postgraduation Agency (CAPES), Ministry of Education, 2005 - 2010
Full-Member, National Academy of Medicine, Brazil, Past President 2015-2017
State University of Rio de Janeiro
Rio De Janeiro, Rio de Janeiro, Brazil