Category: Clinical Oncology: Outcomes & Complications

MP18-20 - Retrospective analysis of the concordance of 20 immunohistochemical tissue markers in metastasectomy lesions in patients with metastatic renal cell carcinoma

Sat, Sep 22
10:00 AM - 12:00 PM

Introduction & Objective :

 This study was aimed at characterizing the different expressions of 20 tissue markers in multiple metastatic lesions and organs in patients with metastic renal cellc acrcinoma (mRCC).


Methods : A total of 66 patients with mRCC, harboring 179 metastasectomy tissue lesions (MTLs), were enrolled with their medical records. Immunohistochemistry analysis was performed for tissue microarrays using 20 tissue markers: BAP1; CD31; CD 34; HIF-1α and -2α; Ki-67; pS6; PBRM1; PDGFRa and b; PD-L1; PSMA; PTEN; α-SMA; TGase2; VEGFR-1, -2, and -3; VHL loss; and CA9. The cases were identified pathologically using the semi-quantitative H-score (0–300). The concordance rate was calculated as the number of patients with concordant intensity score out of the total number of patients in that comparison


Results :

A total of 66 patients were divided into those with the same organs and those with different organs. Forty-two patients (44 cases) with 96 MTLs and 39 with 83 MTLs were used to examine the concordance of the markers within the same and different organs, respectively. Among 179 MTLs, 84 from the kidneys, 43 from the lungs, 25 from the bone, 7 from the brain, 5 from the lymph nodes (LNs), and 15 from other organs were stained with tissue markers. Within the same organs, the BAP1, PSMA, VEGFR3, PDGFRa (>0.9 concordance rate), PS6, HIF-2 (>0.8 concordance rate), PD-L1, and HIF-1 (>0.7 concordance rate) exhibited highly concordant expressions, even though they originated from different MTLs. As for the expression of different metastatic organs in the same patient, BAP1, PSMA, HIF-1, VEGFR3 (>0.9 concordance rate), PDGFRa, SMA (>0.8 concordance rate), PS6, PD-L1, and PTEN (>0.7 concordance rate) showed consistent results, despite being obtained from different organs. For 26 patients who exhibited recurrence within the same organ, the tissue samples were re-stained for the markers, and MTLs were analyzed to examine the concordance of the recurrent site within the same organ. BAP1, PSMA (>0.9 concordance rate), PS6, VEGFR3, PDGFRa (>0.8 concordance rate), PTEN, and HIF-2 (>0.7 concordance rate) showed concordance. However, there was a limitation in that the distribution of some of the high concordant markers had shifted to one side.


Conclusions :

The high-concordance tissue markers, including BAP1, VEGFR3, PDGFRa, and PSMA (PS6: concordance rate >0.7), showed a similar expression of markers regardless of metastatic organs, metastatic sites, or recurrence, whereas other markers were influenced by other factors.

Sung Han Kim

Assistant professor
Department of Urology, Prostate Cancer Center, National CanceNational CAncer Centerr Center, Goyang, Korea
Goyang, Kyonggi-do, Republic of Korea

Weon Seo Park

Department of Pathoogy, Prostate Cancer Center, National Cancer Center, Goyang, Korea
Goyang, Kyonggi-do, Republic of Korea

Eun Young Park

Biometrics branch, Research institute and National Cancer Center, Goyang, Korea
Goyang, Kyonggi-do, Republic of Korea

Jungnam Joo

Biometrics branch, Research institute and National Cancer Center, Goyang, Korea
Goyang, Kyonggi-do, Republic of Korea

Jinsoo Chung

Department of Urology, Prostate Cancer Center, National Cancer Center, Goyang, Korea
Goyang, Kyonggi-do, Republic of Korea