High-Definition Biotechnology
Paul Blainey
Associate Professor of Biological Engineering and Core Member
MIT Department of Biological Engineering and Broad Institute of MIT and Harvard
Microfluidics and whole-genome amplification are enabling single-cell genomic analyses. At the same time, these technologies limit single-cell genomic studies by imposing cost and complexity (microfluidics) and degrading data quality (whole-genome amplification). Here I will present two new methods for single-cell genome analysis, one that requires no microfluidics or specialized equipment for direct single-cell genome amplification and another that leverages culture-based amplification rather than biochemical amplification to enable studies of de novo mutations in single cells.
