Exhibitor Tutorial

Discovery and screening of protein biomarkers with the FirePlex Technology Platform

Tuesday, February 6
9:30 AM - 10:15 AM
Location: Room 1A

In patients and animal models, molecular biomarkers are used as indicators of normal and pathogenic processes. In drug discovery and screening pipelines, molecular biomarkers are used to assess the mechanism of action, efficacy, and toxicity of lead compounds. To address the need for rapid and sensitive quantitation of protein biomarkers from biological samples, we have developed the FirePlex® Technology Platform. Utilizing patented FirePlex hydrogel particles and a three-region encoding design, FirePlex Immunoaassays allow for true, in-well multiplexing, providing flexible and customizable quantification of analytes in a high-throughput assay format.
Here we present data from studies investigating cytokine profiling in human and rodent samples using our standard-throughput FirePlex immunoassays, which enable quantitation of up to 75 protein analytes per well, and are run in 96well plate format.
We also introduce the simplified workflow of our high-throughput FirePlex (FirePlex®-HT) immunoassays, which enable quantitation of up to 10 protein analytes per well in 384well plate format, and we provide data demonstrating assay performance quantifying key cytokines in multiplex. Together, this novel combination of multiplexed, high-sensitivity assays from FirePlex enables rapid quantitation of protein biomarker signatures in biofluid specimens.

Elnaz Atabakhsh

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Dr. Amy Perea

Manager, Immunoassay Platform R&D
Abcam

Amy Perea received her PhD from Rockefeller University where she worked in Thomas Sakmar’s lab and was a member of the Tri-Institutional PhD Program in Chemical Biology. She then joined Genzyme, a pharmaceutical company, as a postdoctoral associate where her work focused on assay development for biomarker studies. She is currently the manager of the FirePlex Immunoassay R&D team at Abcam, which is responsible for evaluating and developing advancements to the FirePlex Immunoassay platform, including the recent development of the high-throughput assay format.

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