Exhibitor Tutorial

Flexible, high-throughput qPCR for gene expression and genotyping analysis using the SmartChip™ Real-Time PCR System

Monday, February 5
2:00 PM - 2:45 PM
Location: Room 4

In this Exhibitor Tutorial, we will present multiple gene-expression and genotyping studies that demonstrate the reliability and flexibility of the SmartChip Real-Time PCR System. This quantitative PCR platform combines the high-throughput nature of microarrays with the sensitivity, precision, and dynamic range of quantitative real-time PCR. The power of the SmartChip system is derived from the 5,184 individual nanowells included in each chip provided in the SmartChip MyDesign Kit. The chips can be configured with 14 different assay and sample arrangements, allowing you to run the experiments you want instead of limiting them to rigid sample and assay formats. The SmartChip system utilizes 100-nl reactions with only 3–10 ng/μl of input required per reaction, which provides 1) the sensitivity needed to eliminate the preamplification step and 2) significant reagent and cost savings over 25-μl reactions in 384-well plates. With the SmartChip system, you can seamlessly switch between dispensing assay reagents and samples into blank chips and dispensing samples into custom, preprinted chips—without the need for revalidation. Run experiments the way you want, while delivering the accurate and consistent results you expect.

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Christopher Sontag

Product Manager I
Takara Bio USA

Dr. Chris Sontag is a senior marketing specialist at Takara Bio USA, Inc. This follows more than 10 years of research experience. A neuroscientist and stem cell biologist by training, Dr. Sontag obtained his Ph.D. from the University of California, Irvine in 2012 and held a postdoctoral research position at the University of California, San Francisco from 2013–2015. In his role at Takara Bio USA, Chris has focused on supporting the qPCR, gene editing, and stem cell product portfolios by developing technical and educational content for web and print. Chris is also responsible for creating and presenting tutorials and informational seminars to support these research areas.

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Flexible, high-throughput qPCR for gene expression and genotyping analysis using the SmartChip™ Real-Time PCR System

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