Exhibitor Tutorial

High-throughput, high-content imaging of physiologically relevant in vitro tissue models

• 

  Sarah Vargas-Hurlston

  Director Product Marketing
  Molecular Devices
• 

  Jos Joore, MSc PhD

  Managing Director
  MIMETAS - the Organ-on-a-Chip Company

There is an urgent need for more predictive models in preclinical drug development. While 3D cell cultures and complex co-cultures can offer more physiologically relevant tissue models, they have been generally associated with cumbersome techniques, low throughput and poor compatibility with established assays. Microfluidic techniques and especially organ-on-a-chip technology, show great potential to enable the ability to scale complex, perfused 3D cell culture to high throughput.

The MIMETAS OrganoPlate® is a microtiter plate based culture platform for complex 3D, perfused (co)-cultures. It harbors up to 96 tissue chips and is fully compatible with liquid handling equipment, high-content imagers and is easily adopted by end-users.

In our tutorial, MIMETAS will present state of the art techniques for developing, assessing and screening of physiologically relevant organ-on-a-chip tissue models using high-content imaging. We will discuss methods for optimizing assessment of morphology and viability in 3D. In addition, we will highlight phenotypic readouts to allow quantitative characterization of complex tissues using high-content imaging with the ImageXpress Micro Confocal system.
Join our tutorial to learn about:

• Development of physiologically relevant organ-on-a-chip tissue models for drug development
• Translatable assays and readouts for the human kidney proximal tubule, central nervous system, colon, liver and blood vessels
• Quantitative characterization of complex phenotypic effects using confocal imaging and 3D analysis

Also, learn more about bringing automated cellular imaging to your personal bench with the unveiling of our new all-in-one image acquisition and analysis system.