Exhibitor Tutorial

The many and varied workflows of cell line development: A comparison of case studies utilizing automation across a diversity of technologies.

Monday, February 5
12:30 PM - 1:15 PM
Location: Room 1A

Establishing a clonally-derived cell line requires the isolation of a single cell and can be accomplished using several methods. Limiting dilution has traditionally been used because of its simplicity and affordability, but is limited in throughput by its inefficiencies. To circumvent the challenge of throughput, dilution of single cells into semi-solid media was established. This method relies on restricting the movement of cells with the use of semi-solid media, which allows a high-density of single cells to be isolated. Additional techniques for single cell isolation include flow sorters and microfluidics-based technologies. However, although these techniques are very similar in their ability to isolate single cells, their unique technologies bring additional challenges and benefits to each workflow they touch. Through a series of cases studies; in this talk we discuss the advantages and disadvantages of several workflows. We also showcase how our Advanced Workflow Engineering Solutions team at Molecular Devices has customized and integrated aforementioned technologies to optimize such workflows.

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Steve Wiltgen

Product Manager for BioProduction Development
Molecular Devices

Steve Wiltgen is the Product Manager for BioProduction Development at Molecular Devices. He obtained his Bachelor of Science degree in Mathematics and Biological Sciences from the University of Nebraska-Lincoln and his Ph.D. in Neurobiology and Behavior from the University of California, Irvine, where his research focus was developing a novel super-resolution imaging technique to look at ion channel function. Steve has been at Molecular Devices for 5 years, first serving as a field application scientist and now as Product Manager.

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Alison Glaser

Applications Scientist - AWES
Molecular Devices

Alison Glaser is the Global Application Scientist for the AWES (Advanced Workflow Engineering Solutions) group at Molecular Devices. She has been with Molecular Devices for over 11 years, with extensive experience in biotherapeutic research involving platforms such as the ClonePix and QPix. Alison focuses on providing customized and integrated solutions for high throughput automation. Alison has a B.S. in Chemistry from Antioch College.

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The many and varied workflows of cell line development: A comparison of case studies utilizing automation across a diversity of technologies.



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Send Email for The many and varied workflows of cell line development: A comparison of case studies utilizing automation across a diversity of technologies.