Biologics Discovery

High-throughput imaging and selection of viable clones in line with single cell sorting improves viability of clones for downstream analysis

Tuesday, February 6
12:00 PM - 12:30 PM
Location: 7AB

An FDA requirement for biologics production is to provide evidence that the host cell line being employed is derived from a single, parental cell (i.e. monoclonal). Conventional techniques for isolating single cells such as limiting dilution and flow cytometry-based methods are significantly limited by process inefficiencies, including low plating densities and low viabilities. These inefficiencies, in turn, reduce the capability to screen for high-producing clones, thereby increasing timelines and costs at early stages of antibody discovery and cell line development processes. Microfluidics-based methods hold promise for improving upon such inefficiencies due to their ability to sort single cells in a low stress environment. Here we present the optimization of a microfluidics-based method—the single cell printing method—for imaging and screening of clones prior to single cell sorting, which significantly improved cell viability compared to other cell sorting techniques.

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Steven Wiltgen

Product Manager
Molecular Devices

My PhD and post-doc focused on the development of a novel super-resolution imaging technique based on TIRF imaging of calcium permeable ion channels. I then served as an Adjunct Professor at several community colleges in the Southern California region before beginning my career at Molecular Devices 4 years ago. I first served as a Field Application Scientist for our high-content imaging products, then switched gears to marketing where I served as an application scientist for our bioproduction development line of products. I now serve as the product manager for these same instruments and have been in this role for 1 year.

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