Category: Automation and High-Throughput Technologies

1044-E - An Automated Method of THP-1 Cell Propagation and Differentiation: Application in the examination of CD33 and TREM2-related signaling pathways associated with myeloid biology

Wednesday, February 7, 2018
11:30 AM - 12:30 PM

Automated methods of cell propagation have been routinely deployed in large scale drug discovery campaigns for a number of years.  Engineered cell lines have been the work horses ofe cell based drug discovery and their use in automated cell propagation widely cited.  The advantages of a centralized, quality controlled, and scheduled automation program for the maintenance and plating of such cells is also well known.  Recently, a shift in enabling biology has facilitated the deployment and use of phenotypically relevant, directed differentiation modalities for discovery.  The need and ability to form relevant germ-line specific phenotypic models for drug discovery has led to advances in liquid handling and automation that accommodate this more complex biology.  Here we describe a turn-key repeating method developed for THP-1 cell propagation and 384 well plate based differentiation to a myeloid fate using the Sartorius CompacT SelectT and advanced plating module.  

Christopher JOHN. Hogan

Senior Scientist
Abbvie
Cambridge, MA

My current expertise is overseeing automated cell culture and iPSC efforts for Abbvies' Foundation Neuroscience Center in Cambridge MA, and helping advance NS efforts here and among our global collaborators. My interests lay in further exploring automated models utilizing induced pluripotent stem cells, 3-D modeling, and primary cells. Past experience includes working with induced pluripotent stems cells, and mesenchymal stem cells. My lab and I successfully reprogramed patient derived cells into IPSC's, and subsequently differentiated these into phenotypicaly relevant cardiomyocytes for discovery efforts. Other directed differentiation strategies included neuronal and hepatocyte fates. Implementation of 3D cell culture and spheroid strategies has also been successful.