Category: Automation and High-Throughput Technologies
Three-dimensional (3D) cell models are thought to better mimic the complexity of in vivo tumors. We have previously enabled an HTS-compatible method using cell-repellent plates combined with a magnetic force bioprinting technology that affords large scale testing of spheroids and organoids in flat-bottom 384 and 1536 well plates. This type of 3D biology requires tissue culture in suspension which makes feeding, media transfers, washing, etc., problematic. To address this we combined the 384 well formatted magnetic 3D (m3D) Bioprinting technology with the utility of the BlueWasher equipped with magnetic spin features. We validated this system for rapid removal and replacement of media to facilitate adaptation of a non-homogeneous format within 3D HTS. Our work demonstrates the effectiveness of both the m3D bioprinting system and the BlueWasher in the following aspects in 384 well plates: 1) Retention of 3D spheroids and their integrity 2) Precision and accuracy of liquid manipulation 3) Qualification in terms of compound controls, Z’ and S:B when using phenotypic 3D primary pancreatic tumor cell based assays.
Shurong Hou– Postdoctoral fellow, The Scripps Research Institute - FL, Jupiter, FL
The Scripps Research Institute - FL
Shurong Hou is a postdoctoral fellow in The Scripps Research Institute Molecular Screening Center, who has dedicated herself to early drug discovery. She is interested in assay development of biochemical and cell-based assays for high throughput screening, especially developing physiologically relevant 3D tumor models for cancer drug discovery.