Category: Automation and High-Throughput Technologies

1369-E - Semiautomated Cell Culture Workflow for iPSC-Derived Human Neurons

Wednesday, February 7, 2018
11:30 AM - 12:30 PM

Drug discovery programs for central nervous system (CNS) disorders having increasingly employed model systems with greater physiological relevance. This strategy is an attempt to reverse high attrition rates seen at all stages of development. Such efforts include the use of neurons derived from human induced pluripotent stem cells (iPSCs). Directed differentiation technology can be used to generate neuronal subtypes from either normal or disease iPSC lines. However, these neurons require specific handling to ensure consistent results. Toward this end, we have implemented a cell culture workflow using human iPSC-derived spinal (lower) motor neurons and a liquid handling system (PIPETMAX). As we illustrate here, the system can be used at all workflow points, including: (1) plate coating and washing, (2) cell seeding, (3) medium changes/additions, (4) conducting cell viability assays, and (5) immunocytochemical staining. This system reduces time spent on repetitive tasks and allows for consistent repetition of cell culture tasks within a complete workflow.

Laura Simdon

Support Services and Training Manager
Gilson, Inc.
Middleton, WI

Dr. Laura Simdon is Support Services and Training Manager at Gilson, Inc., creator of PIPETMAN and a wide range of laboratory robotics. She leads a team of scientists responsible scientific applications, training, technical support and service support at Gilson. The team interacts with new product development and marketing throughout the technology commercialization workflow. Dr. Simdon has a background in molecular biology and metabolic engineering and is experienced with laboratory robotics (automated liquid handling, HPLC, SPE, NGS library prep). She holds six U.S. patents and earned her Ph.D. in Biology from the Massachusetts Institute of Technology and a B.S. summa cum laude from the University of Minnesota-Twin Cities, with a major in Genetics and Cell Biology and a minor in Speech-Communications.