Category: Automation and High-Throughput Technologies

1311-B - Fluorescence measurement of Ca2+ transients in human iPSC-derived cardiomyocytes for assessment of drug-induced proarrhythmia: Automatic detection of EAD-like waveforms

Monday, February 5, 2018
5:00 PM - 6:00 PM

Human iPSC-derived cardiomyocyte (hiPSC-CM) is considered a novel promising tool in cardiovascular disease research and cardiac safety pharmacology. Ca2+ transient is a key phenomenon in the excitation-contraction (EC) coupling in cardiomyocytes and the fluorescent measurement of Ca2+ transients with calcium-sensitive fluorescent dyes in hiPSC-CMs has been used to detect drug-induced cardiotoxicity such as arrhythmic risks and contractility changes. In the assessment of arrhythmic risks of compounds from Ca2+ transients measurements, the changes in Ca2+ transients-waveforms are analyzed. In the analysis of Ca2+ transient waveforms measured with a kinetic plate reader FDSS, the FDSS Waveform Analysis Software gives various parameters’ values such as peak rate, peak amplitude, peak to peak time, peak width duration (PWD)(10%~ 90% with 10% step), and area under curve. It also calculates the rising and falling slopes using 10%-90%, 20%-80%, or 30%-70% of PWDs. Furthermore, we have been developing an “irregular peak detection” function for detection of early afterdepolarization (EAD)-like waveforms automatically, which is important  in arrhythmic risk assessments using hiPSC-CMs. We compared the results of EAD-like waveform detection by our method in the FDSS Waveform Analysis Software with those by the visual check.

Natsumi Kato

Application Engineer
Hamamatsu Photonics K.K.
Hamamatsu City, Shizuoka, Japan

Natsumi Kato is an application engineer at Hamamatsu in Japan since 2011. She studied Molecular Biology at the Nagoya University before joining Hamamatsu, and obtained her PhD degree in Photonics Industry Management from the Graduate School for the Creation of New Photonics Industries in 2016.