Category: Assay Development and Screening
The Compound Management (CM) team within St. Jude Children’s Research Hospital’s Chemical Biology and Therapeutics Department was tasked with acquiring, registering, and reformatting an in-house comprised list of commercially available compounds approved by the Food and Drug Administration (FDA). The compounds were sourced over a period of 15 months, and, using a Pipeline Pilot web application, registered into our Perkin Elmer ChemBioOffice Registration Enterprise database. Once registered, these samples were added into our Titian Mosaic Sample Management System for tracking and storage. Each compound was then weighed, solubilized, filtered, and transferred into a 96-well “source” plate. Next, an aliquot was delivered to chemists in our Analytical Technology Center (ATC) for qualitative analysis using CLND, and purity analysis using both UV-Vis and ELSD. The source plates were then reformatted, using a Tecan EvoWare Multi-Channel Arm (MCA) and Liquid Handler Arm (LiHa), into 4 different destinations: 384-well screening plates, 384-well REMP STbR plates, 384-well Labcyte Echo plates, and 384-well Dose Response Plates. The completed plates were then placed either into our REMP MSS, or stability chamber until they are requested by researchers. Remaining source plate solutions were stored for regeneration of any consumed destinations. Titian’s Mosaic was utilized heavily during this process enabling real-time labware and sample definition, tracking, and processing from beginning to end, allowing CM generate readily available screening material to meet a variety of research needs.
Parker Hemphill– Compound Management Assistant, St. Jude Children's Research Hospital, Germantown, TN