Category: Assay Development and Screening
The Small Molecule Screening Facility (SMSF) at the University of Wisconsin Madison is a comprehensive high throughput screening (HTS) center that offers services to academic and industry clients worldwide. The facility has an in-house chemical library of >450,000 compounds that is available for screening to our customers. Compounds are delivered is via acoustic liquid handling using a Labcyte Echo 550 instrument.
Here we present two examples of HTS in 1536 well plates, a screen for protein-protein interactions and a cell based luciferase reporter assay. We will present methodology for assay miniaturization and optimization in 1536 well format. We will discuss the impact of the above on academic HTS endeavors at our facility.
Gene Ananiev– Reserach Scientist, UW Madison, SMSF, Madison, WI