Category: Automation and High-Throughput Technologies
The GNF Systems Washer-Dispenser II (WDII) is a high precision instrument for reagent dispensing and micro-titer plate washing (aspiration). Regular use of the WDII for plate washing in high throughput screens produces large amounts of fluid waste. The WDII standard configuration provides a 20 liter waste container which is ample for most standard applications. The WDII waste carboy is under vacuum allowing aspiration or wash waste to be actively managed. However, this vacuum prevents facile and timely draining of the carboy under operating conditions. Many high content imaging screens require multiple wash cycles per plate. When these projects are executed over hundreds of plates the stock waste container cannot provide enough capacity. GNF has developed a system to manage the liquid waste of the WDII to drain continuously so that large, wash intensive screens can be performed after hours unattended. This poster will describe the development strategy and end results of the automated waste management mechanism for the WDII.
Jason Matzen– Automation Engineer, GNF Systems, San Diego, CA
San Diego, CA
As an automation engineering with fifteen years of industry experience, Jason Matzen has worked with HTS automation systems his entire career. Starting as a quality control specialist at Aurora Biosciences he learned the landscape of the biotech industry. He transferred to the Genomics Institute of the Novartis Research Foundation (GNF) in early 2002 to run their HTS system. Jason has worked on a variety of screening projects including reporter, target, and phenotypic screens. He has since transitioned to oversee the screening operations at GNF and work with GNF’s engineering group to develop new instrumentation.