Category: Assay Development and Screening
All instruments, including multimode readers, emit heat from mechanical and electronic components. This heat can affect the reaction kinetics of samples being investigated inside the reader. The Tecan Spark® plate reader with Te-CoolTM module allows adjustment of the instrument internal working temperature from 18°C to 42°C. The instrument also includes a mode to set the internal temperature at the ambient temperature of the room. Having the ability to investigate samples of interest at ambient temperature, can limit heating effects on enzymatic reaction dynamics. In this work we present the benefits of an actively cooled multimode reader in investigations employing bioluminescence reactions, kinetic profiling and high throughput screening.
The kinetic profile of enzymatic reactions can appear very different when acquired on readers that are equipped with an internal cooling capability versus instruments that do not have this functionality. We also show that bioluminescent assay-based compound library screens results can be significantly distorted due to the internal heating of plate readers.
We also show that bioluminescent assay-based small compounds library screens results can be significantly distorted due to the plate reader’s internal heat effect on bioluminescent assay
GEDIMINAS VIDUGIRIS– Senior Automation Scientist, Promega Corporation, Madison, WI
Senior Automation Scientist
Gediminas Vidugiris is an Senior Automation Scientist at Promega Corp. Madison, WI.
He received his PhD in Biochemistry at Institute of Biochemistry, Lithuania Academy of Science, Vilnius, Lithuania. Dr. Vidugiris has extensive multidisciplinary research and development experience in systems automation, screening, assay development, biochemistry, biophysics, spectroscopy and electrochemistry.