Category: Automation and High-Throughput Technologies

1249-E - A microfluidics-based single cell isolation workflow optimized for efficiency, viability, and assurance of monoclonality

Wednesday, February 7, 2018
11:30 AM - 12:30 PM

Cell line development is a crucial step in upstream processes for generating biopharmaceutical molecules like monoclonal antibodies. Traditional workflows in cell line development have major drawbacks such as inefficient single-cell isolation, reduced cell viability, and limited evidence of monoclonality. We present an approach to address these challenges by utilizing a microfluidics-based single-cell isolation technology and a high-throughput imaging system. The CloneSelect™ Single-Cell Printer™ offers documented proof of clonality and provides efficient and fast single-cell seeding combined with excellent cell viability and zero risk of cross-contamination. Meanwhile, the CloneSelect™ Imager allows for fast and objective confirmation of monoclonality as well as the growth characteristics of single cell-derived clones. The two systems can be used in concert to increase throughput with the confidence of clonality and objective analysis of clones for downstream steps in the cell line development workflow.

Sarmad Al-Bassam

Applications Scientist
Molecular Devices
San Jose, California

Sarmad is an applications scientist in BioProduction Development at Molecular Devices. Sarmad holds a PhD in Molecular Biology and has over 10 years of life science bench experience as well as a cross disciplinary training in technical sales and applications. After completing his postdoctoral training he was a Field Applications Scientist for cellular imaging microscopy and plate reading. Through his career in industry, he has held roles ranging from developing emerging applications to sales, product development and customer support. In his free time he enjoys running, hiking in the outdoors, reading and traveling.