Category: Automation and High-Throughput Technologies
Every assay, whether for screening, hit to lead or lead optimisation, requires significant experimental optimisation for robust assay performance. Variables requiring optimisation can include things like antibody concentrations, bead number, buffer constituents, pH, as well as a variety of incubation time points. However, with a manual approach the number of parameters that can be varied is often limited due to the sheer difficulty and time it would take to dispense all the components in the desired combinations, leading to long and iterative assay development cycles.
Here we have used a novel positive displacement dispensing instrument to reduce assay development times to convert components from a commercially available ELISA kit onto a bead to perform much more simple no-wash multiplexed ELISA assays. Parameters such as antibody conjugation protocols onto the beads have been investigated to optimise the loading of target antibody, in addition to investigating different buffer components and concentrations of detection antibodies and fluorescent labels.
In this poster, we demonstrate a no-wash 5-plex cytokine assay in a 384 well plate that has been converted from a standard ELISA plate based format by using the dragonfly discovery automated pipettor.
Joby Jenkins– Director, Product Strategy, TTP Labtech, Royston, England, United Kingdom
Director, Product Strategy
Royston, England, United Kingdom
Joby's role combines engineering expertise, project, product and account management to aid the design, development and sales of innovative products and solutions for healthcare, pharmaceutical and biotechnology sectors. With a first class honours degree in product design and manufacture from Loughborough University, he has 10 years experience in industrial research and development, project management and sales and marketing. Since joining Labtech, he has been a key engineer and project leader for the development of the mosquito® range of products, which provide precise nanolitre liquid dispensing with zero cross contamination, and has been instrumental in establishing TTP Labtech as an internationally recognised provider of leading-edge technologies for drug discovery and development.