Category: Chemical Biology
Integrity of a pharma’s small molecule collection is hallmark to its Compound Management group. Chemical stability of any small molecule compound can be affected by various environmental conditions during storage. This includes, but is not limited to, the physical state of the stored compounds. A preliminary study was carried out to compare the effects of long-term ( ≥ 15 years) storage of compounds, dissolved at 4mM concentration and frozen in DMSO at -20°C within the Pfizer Liquid Store as compared to the same compounds stored dry at the Pfizer Solid Store. A set of compounds was selected for the study based on availability in both the liquid and dry libraries as well as the age of the compounds, with oldest compounds being chosen. Identical batches were chosen for all comparisons. Structural diversity and functional group representation was also retrospectively considered when reviewing the results. Compound relative purity and identification were confirmed with liquid chromatography/ultraviolet spectroscopy/mass spectrometry (UPLC-MS) analysis. Comparison of relative purity was drawn between compounds stored as solids and as liquids. The observed purity values suggest that the differences in stability between compounds stored as liquids versus neat were indistinguishable. Compounds were found to be comparably stable or degraded irrespective of whether they were stored as solid or as liquids.
Robert Mongillo– Scientist, Pfizer, Guilford, CT