Category: Micro- and Nanotechnologies

1032-C - Acoustic Droplet Ejection of Aqueous Solutions from an Acoustic Tube - Enabling qPCR

Tuesday, February 6, 2018
2:00 PM - 3:00 PM

     Acoustic droplet ejection (ADE) is a fluid dispensing technology, whereby high-frequency sound waves are focused on the surface of a fluid to eject nano- and picoliter volumes with high accuracy and precision. We have previously developed this technology to transfer drops from wells of 384 and 1536 micro-titer plates, over a wide range of fluids. By using the new Echo® Qualified Acoustic Tube with the Echo 655T, we transfer 2.5 nL drops from an acoustic tube filled with aqueous fluids exhibiting surface tension values ranging from 72 mN/m for phosphate buffered saline (PBS) to 30 mN/m for PBS with 0.043% v/v Triton X-100. Each tube can be individually accessed and decapped, enabling customizable workflows for life science applications that were previously not possible. Tubes have a screw-cap design, allowing for secure, long-term storage of precious or rare samples for later use and eliminating the need for disposable tips or intermediary plates.

      We transferred 0.5 µL of human genomic DNA (0.78 to 100 ng/µL) from an Echo Qualified Acoustic Tube using an Echo 655T. Reagents were transferred with accuracy and CV values less than 5%, demonstrating the efficacy of this consumable for life science applications. This performance enables us to perform a quantitative real-time polymerase chain reaction gene expression assay using the housekeeping gene, β-actin. These results demonstrate that the Echo 655T, in combination with the acoustic tube, provide improved throughput across a wide range of reagents, particularly for applications that require miniaturizing biochemical assays.

Carson Riche

Sr. R&D Engineer
Labcyte
San Jose, CA

PhD Chemical Engineering