Category: Automation and High-Throughput Technologies
A high quality genomic DNA preparation is essential for downstream applications. Consistency in isolating high quality DNA while eliminating cross-contamination of samples has long been a pain point of manual DNA isolation approaches. Automation of DNA purification by using DNA-binding magnetic methodologies, in combination with, an automated liquid handling system can increase yields while eliminating cross-contamination. This recovered DNA can then immediately be used for downstream applications, including; PCR and DNA sequencing. Here we evaluate the automation of DNA purification from blood using the Versa 1100 Nucleic Acid Purification and PCR Setup Workstation from Aurora Biomed Inc. Aurora’s AB96 Magnetic Bind Blood DNA Isolation kit was used to isolate genomic DNA from pig’s blood in a 96-well format. High, sharp molecular weight DNA bands were detected by agarose gel electrophoresis. The concentration of isolated gDNA was consistently 2-fold higher (60.0-80.0 ng/µL) for DNA isolated by automation compared to the manual bead isolation.Further qualification of the extracted samples was completed by using the purified samples as templates for the amplification of β-actin. Efficient recovery of DNA was indicated by the lack of detectable nucleic acids in both the wash fluids and a second elution. Automation of the DNA extraction methodology facilitated efficient, high-throughput isolation of high quality DNA from blood samples that can be immediately used for downstream applications, in this case, PCR. This data clearly demonstrate the advantages of automating routine DNA purification and downstream applications.
Grant McNair– Product Specialist, Aurora Biomed Inc, Vancouver, BC, Canada