Category: Assay Development and Screening
The prevalence of three dimensional (3D) cell culture is increasing in pharmaceutical and biotechnology research as the advantages of the more physiological 3D models relative to traditional two dimensional (2D) cell culture become evident. The Corning microcavity vessel platform was developed as a tool for customers working with 3D cell aggregate models in order to easily scale-up their 3D culture production. The substrate of microcavity vessels is a polystyrene film formed to have 157 gas-permeable micron-sized wells (referred to as “microcavities”) per square centimeter (cm2). The microcavities are approximately 500 micrometers (µm) in diameter and 600 µm in depth and have an Ultra-Low Attachment surface. The Ultra-Low Attachment surface, rounded well-bottom geometry and gravity encourage the growth of cells as aggregates. The nature of the microcavity vessel as a single vessel means that cell aggregates can be grown in bulk and importantly, all are cultured in identical conditions. Once generated, there is the need for down-stream sorting and dispensing of these 3D aggregate cultures for further applications. For the sorting and dispensing of objects that, because of size or fragility, cannot be processed by current automated methods, Union Biometrica has developed specialized automated flow cytometry instruments. Accordingly, we describe a collaboration between Union Biometrica and Corning for an in-house evaluation of the BioSorter® Large Particle Flow Cytometer for sorting, based on size and fluorescence, and dispensing of 3D cell aggregates that had been generated in bulk format in microcavity vessels.
Hilary Sherman– Applications Scientist, Corning Incorporated, Kennebunk, ME
Hilary Sherman is an Applications Scientist in the Corning Life Sciences Lab located in Kennebunk, ME. Hilary has been with Corning Incorporated for 12 years and has worked with a wide variety of cell types including mammalian, insect, primary and stem cells in a vast array of applications. Her key roles at Corning involve creating technical documents such as protocols and SNAPPshots and providing technical support and training for both the Corning sales force as well as customers. Hilary received her B.S. degree in Biology from the University of New Hampshire in 2005.