Category: Assay Development and Screening

1189-E - MALDI-TOF sample preparation - simultaneous 1536 well pipetting enables next generation drug discovery screening

Wednesday, February 7, 2018
11:30 AM - 12:30 PM

Introduction


In biopharmaceutical laboratories there is a growing need for label free high-throughput screening assays based on mass spectrometry. Traditional MS based techniques have been relatively slow and thus have not suited for high throughput screening (HTS – 10,000 compounds/ day) or ultra HTS (uHTS – 100,000 compounds/ day).Matrix-Assisted Laser Desorption/ Ionization Time-of-Flight (MALDI-TOF) MS provides an alternative format for HTS/uHTS of small molecules, peptides and proteins for enzyme assays. Providing analysis times ~0,25 second/sample and nanoliter volumes, this technique offers a MS format with throughput and sample volumes closer to traditional HTS/uHTS.


 


Methods


Analytik Jena and its collaboration partner Bruker built a bridge between the powerful and unique label free detection capabilities of mass spectrometry and automated uHTS MALDI sample preparation. With the pipetting device CyBio® Well vario 1536 including its accessories Analytik Jena offers more than just a spotting device which works in concert with the new rapifleX® by Bruker and therefore overcomes application specific bottlenecks. The presentation will give an introduction about our automation solution which can prepare 1536 MALDI sample plates within the same time the mass spectrometer can measure them. The liquid handling workstation operates the whole workflow for sample deposition on MALDI sample plates, MALDI matrix spotting and active drying. In addition the pipettor offers a desalting procedure called on-target-washing in order to remove undesired components to increase the system performance without losing time.


 


Results


The fully automated dispensing and analysing system shows an unreached performance in drug discovery screening and early drug development and completes a 2 million molecule diversity screen within 10 days.


 


Conclusion


The presentation will show how liquid handling automation in drug discovery screening can overcome application bottlenecks and lead to a breakthrough in throughput and assay robustness and reliability.

Swen Tyrasa

Head of Automation
Analytik Jena AG, Jena, Germany
Jena, Thuringen, Germany

Automation