Category: Automation and High-Throughput Technologies

1216-B - Quantifying double-stranded DNA with fluorescent dyes: Qubit™ on BMG LABTECH microplate readers

Monday, February 5, 2018
5:00 PM - 6:00 PM

Next generation sequencing (NGS) has become an essential tool in basic research, diagnostics and forensics. The modern sequencing techniques not only provide a high reading depth and speed but also paved the way for processing a higher amount of samples. A prerequisite for reproducible sequencing results is the precise determination of DNA before library preparation and sequencing. The gold-standard for DNA quantitation prior to NGS workflows is a fluorescent DNA dye: Qubit™. Here, we present the transfer of the single-tube Qubit™ DNA quantification method to a microplate and analysis with fluorescence microplate readers. Fluorescent DNA quantification with Qubit™ in a 96-well microplate kept the sensitivity and simplicity of the assay while providing higher throughput and rapid replicate measurements. Hence, the microplate-based Qubit™ measurement fills the gap between increased sample number and single sample DNA quantification.

Tobias Pusterla

International Marketing Manager
Ortenberg, Baden-Wurttemberg, Germany

Tobias Pusterla is International Marketing Manager with BMG LABTECH. He graduated in Veterinary Biotechnology at the University of Milan, Italy. After an experience in the Core Facility for Mouse Mutagenesis in Milan, he obtained a Ph.D. in Cellular and Molecular Biology at the Open University of London, UK, in a joint program with the Hospital and Scientific Institute San Raffaele, Milan, Italy. Subsequently, he joined as a postdoc fellow the German Cancer Research Center (DKFZ) in Heidelberg, Germany. He joined BMG LABTECH in January 2013.