Category: Automation and High-Throughput Technologies

1193-D - Sensible High-throughput Automated Cloning

Tuesday, February 6, 2018
5:00 PM - 6:00 PM

The Saccharomyces cerevisiae cell based assay known as MYTH (membrane yeast two hybrid) allows for the interrogation of numerous Schizosaccharomyces pombe protein-protein interactions embedded in, or associated with, cellular membranes, but requires the construction of thousands of bacterial expression clones. To achieve this, the Automation group within the Stowers Institute’s Molecular Biology Core has developed a high-throughput workflow utilizing liquid handling robotics, as well as automated colony picking, to quickly and reliably generate vectors containing Pombe coding sequences via a ligation independent Gibson assembly method. Combined with miniaturized chemistry and automated data analysis, our liquid handling robots can economically produce hundreds of bacterial clones in a matter of days.

Dan Bradford

Lab Manager II
Stowers Institute
Kansas City, MO

I am William Bradford, Laboratory Manager II, of the Stowers Insitute for Medical Research in Kansas City, Missouri. Ever since joining the automation group within the Molecular Biology Facility at the Stowers Institute I have been interested in migrating and expanding assays typically performed at the bench with single channel pipettes onto large, multi-armed, liquid handling robots. Through countless cross collaborations over the years we have successfully helped many labs and institute members achieve their automation potential.

Dan Bradford

Lab Manager II
Stowers Institute for Medical Research