Category: Advances in Bioanalytics and Biomarkers

1178-D - Fluorescent Quantification of low amounts of dsDNA using AccuBlue NextGen from Biotium

Tuesday, February 6, 2018
5:00 PM - 6:00 PM

Sensitive quantification of DNA in a sample is of vital importance to applications such as forensic DNA analysis where low amounts of DNA may be present. Similarly, accurate DNA quantification is paramount for Next Generation Sequencing (NGS), where precise amounts of DNA are required to enable this powerful tool in the investigation of genetic phenomena involved in mutations that lead to cancer or disease. Fluorescent quantification methods have proven accuracy and excellent sensitivity. Here we show the performance of DNA quantification using AccuBlue™ NextGen reagent with detection by the CLARIOstar® microplate reader. It has the sensitivity to enable optimal performance of the AccuBlue NextGen dsDNA Quantification Kit. We obtained excellent linearity across all DNA concentrations tested and observed LOD values in 96 and 384 well plates that are better than previously reported. Thus this combination provides excellent sensitivity in DNA-quantification and the ability to conserve samples when needed.

Maggie Nakamura

Sales Manager
Cary, NC

Maggie Nakamura is the Northern California and Pacific Northwest Sales Manager for BMG Labtech. She has a B.S. in Biochemistry and Molecular Biology from University of California, Santa Barbara. Maggie has been a sales professional in the Life Sciences industry for over 21 years including 11 years with BMG Labtech.