Category: Biologics Discovery

1033-D - A Fully Automated Microscale Transient Transfection System

Tuesday, February 6, 2018
5:00 PM - 6:00 PM

A Fully Automated Microscale Transient Transfection System

Allison Gablin
Research Associate, Early Stage Cell Culture
Genentech, Inc.

Transient transfection of mammalian cells provides a rapid method of producing recombinant protein for various research purposes. We use automation to complete high throughput transient transfections at the 1 mL scale in 96 well plates. A semi-automated system has previously been used for 1 mL transfections, but a significant increase in demand for 1 mL expressions prompted the goal to develop a fully automated method to increase execution efficiency. Our robotic system consists of a Tecan Freedom Evo® 200 integrated with a LiCONiC LPX220 plate hotel, two LiCONiC STX44 incubators, a Multidrop™ Combi, and a KAPS 500 plate sealer. Prior to creating the fully automated method, we optimized cell culture parameters to support cell seeding into 1 mL plates via the Multidrop. Additionally, multiple rounds of testing and extensive redesigns of the LiCONiC automated incubator were necessary to build a functional instrument. A fully automated transfection method on the Tecan EVO® capable of transfecting 18 x 96 well plates in 2 hours was scripted, tested and shown to produce comparable titers to manual transfections.

Allison Gablin

Research Associate
Genentech
South San Francisco, CA

I have a BS in Biology from the University of California, Santa Cruz and an M.S. in Biotechnology from Johns Hopkins University. I currently work in the Early Stage Cell Culture group at Genentech Inc. I have 4 years of cell culture experience, 3 of those years focusing on automation and liquid handling.