Category: Cellular Technologies
Identification of small molecules that control neural stem cells (NSCs) fate would be helpful to facilitate the development of therapeutic applications. In the current study, we developed and screened small molecules that can modulate the NSC fate that are derived from rat fetal cortex. Among these compounds, compounds 5 and 6 successfully differentiated NSCs into astrocytes and neurons, respectively. Compound 5 induced astrocytogenesis by increasing expression of interleukin-6, bone morphogenetic protein 2 and leukemia inhibitory factor and through consequent phosphorylation of signal transducer and activator of transcription 3 and Sma- and Mad-related protein 1/5/8 in NSCs. In addition, compound 5 increased the expression of fibroblast growth factor (FGF) 2 and FGF8 which may regulate the branching and morphology of astrocytes. Taken together, our results suggest that these small molecules can serve as a useful tool to study cell fate determination in NSCs and be used as an inexpensive alternative to cytokines to study mechanisms of astrocytogenesis.
HYUN-JUNG Kim– Professor, Chung-ang University, Seoul, Seoul-t'ukpyolsi, Republic of Korea
Seoul, Seoul-t'ukpyolsi, Republic of Korea
My research area is neural stem cells (NSCs). I have been studying how to control NSC fate, mainly. In my lab, I and my lab members are screening small molecules and natural products to identify potential reagents or drugs that regulate NSC fate. In addition, we do study how NSC fate is regulated. Recently, we have published MAPK, STAT, and epigenetic regulations including histone demethylases are important playeers that control NSC fate.