Category: Cellular Technologies
Skin and lung are some of the specialized barrier tissues used in in-vitro test systems in predicting and providing dependable pre-clinical research data. Three-dimensional (3D) cell culture systems aim to provide cells from these barrier tissues with a more natural growth environment with the help of e.g. hydrogel matrixes or synthetic scaffolds. Collagen, in particular collagen type I, is one of the most abundant extracellular matrix proteins in the body and therefore an often used 3D cell culture material. Lonza offers the novel RAFT™ (Real Architecture for Tissue) 3D Cell Culture system that allows the creation of tissue-like structures with cells growing within or atop a high-density collagen scaffold. We present data here demonstrating differences in a lung co-culture model in two dimensional (2D) and in the RAFT™ 3D cell culture system using Clonetics™ normal and asthmatic bronchial epithelial cells and smooth muscle cells. The cell proliferation, morphology, growth factors and cytokines were investigated for both 2D and 3D systems. We also demonstrate full thickness skin using Clonetics™ keratinocytes and fibroblasts in the RAFT™ 3D cell culture system. Evaluation by histology and immunofluorescence markers confirmed the resemblance to native skin. These 3D cell culture system models provide a valuable tool to investigate the barrier-tissues in-vivo like collagen based microenvironment potentially allowing pre-clinical testing of drug efficacy and safety.
THERESA DSOUZA– Section Manager, Cell Biology R&D, Lonza Walkersville Inc., Walkersville, MD
Section Manager, Cell Biology R&D
Lonza Walkersville Inc.
The environmental cues cells are experiencing in a three-dimensional (3D) cell culture environment bring them closer to their in-vivo state compared to two-dimensional (2D) culturing surfaces. Lonza offers the novel RAFT™ (Real Architecture for Tissue) 3D Cell Culture system that allows the creation of tissue-like structures with cells growing within or on top of a compressed, high-density collagen scaffold. The created dense scaffold allows investigation of several cell processes.