Category: Automation and High-Throughput Technologies
Polydimethylsiloxane (PDMS) has been widely used in fabricating microfluidic devices for prototyping and proof-of-concept experiments. Due to several material limitations, PDMS has not been widely adopted for commercial applications that require large-scale production. This paper presents a mass-producible Injection-Molded Plastic Array for 3D Culture Tissue (IMPACT) platform that incorporates a novel microfluidic design to integrate patterned 3D cell culture within a single 96-well. Multiple hydrogel patterns can be sequentially assembled by utilizing capillary-guided flow along wedges and narrow gaps designed within the well. The patterning is quick and robust. It is as simple as placing a liquid droplet on any part of the wedge structure to obtain spontaneous patterning within 1 second. Optimal dimensionless parameters required for successful capillary loading have been determined. The IMPACT platform was used to co-culture HUVEC and LF, and to obtain angiogenic sprouts with lumens and tip cells that are comparable to those observed in PDMS based devices. A key feature of this platform is the ease of use and efficiency in terms of time, and cost for 3D co-culture. Moreover, it works at most stages such as fabrication, experiment preparation, and cell arrangement. We expect that the injection-molded PS platform with novel capillary filling design can be used in building organ-on-a-chip platform that is compatible with imaging and fluid handling equipments used in high-throughput screening. This platform is expected to have a wide range of applications related to biological discovery, tissue engineering and drug screening, and can be used in a variety of scales from lab bench to automated equipment.
Younggyun Lee– MS candidate, Division of WCU (World Class University) Multiscale Mechanical Design, Seoul National University, Seoul, Seoul-t'ukpyolsi, Republic of Korea
Division of WCU (World Class University) Multiscale Mechanical Design, Seoul National University
Seoul, Seoul-t'ukpyolsi, Republic of Korea
Mechanical Engineering, Manufacturing, Bio-MEMS, Micro fabrication, Mechanical Design, Senior engineer of Samsung Electronics Health and Medical Equipment Division