Category: Cellular Technologies

1061-B - HTP single cell library preparation using acoustic handling

Monday, February 5, 2018
5:00 PM - 6:00 PM

The advent of single-cell sequencing technologies enabled a new era of biological research. Researchers aim to examine 1,000s of cells to understand the composition and function of cell populations and how individual cells contribute to the greater picture of tissue and organ function, development and disease. The Wellcome Trust Sanger Institute has established a Single Cell Genomics Core Facility to support this research. An essential part of this facility is a miniaturised and fully automated Nextera library preparation workflow. By using an ECHO525 acoustic dispenser and integrating it with 3rd party devices we have developed a fully validated workflow that is able to quantify and normalise samples before library preparation in sub-┬Ál volumes. The whole system is run in an autonomous fashion that requires minimal user intervention and is able to deliver up to 4000 full-length cDNA or whole genome sequencing libraries per day in a cost-effective way.

Stephan Lorenz

Head of Single Cell Genomics R&D
Wellcome Trust Sanger Institute
Cambridge, Hinxton, England, United Kingdom

I acquired my PhD at the University of Leipzig investigating mutational processes in the thyroid elicited by endogenous hydrogen peroxide synthsis. I joined the the Wellcome Sanger Institute in 2013 as a PostDoc for single-cell genomics method development. In 2014 I started to implement and run a high-throughput single cell RNA & DNA sequencing service platform, exploring how single cell methods could be delivered at scale through innovative automation platforms. Since 2017, I am focussing on improving existing single cell methods and implement a wider range of methods into the service platform at the Wellcome Sanger Institute, like 10X Genomics droplet systems or combinatorial indexing methods. Further, I am managing the bespoke library preparation and long read group, where I am driving high-throughput delivery of ChipSeq, 10X Genomics linked reads and PacBio long read sequencing services.