Category: Advances in Bioanalytics and Biomarkers

1435-A - Development of LC-MS/MS method for measuring dopamine and its metabolites in Drosophila melanogaster model of Parkinson's disease

Monday, February 5, 2018
2:00 PM - 3:00 PM

The need for implementation of 3Rs principles (replacement, refinement and reduction) in animal experiments has been well acceptedby the scientific community across the globe. Drosophila melanogaster (Fruit fly) model is consider to be one the replacement modelfor rodents and other higher animals used as Parkinson's disease model. In addition to being less sentience, the model is inexpensiveand genetically homogeneous. In the present study we have developed and validated a LC-MS/MS method for estimating dopamineand its metabolites in Drosophila head and body samples. The developed method is rapid, reliable, reproducible and sensitive todetect the biological levels of dopamine and its metabolites. The LC-MS/MS method developed for the estimation of dopamine andits metabolites in Drosophila melanogaster has wide application in screening antiparkinson's/ neuroprotective agents based on thedopamine and its metabolites levels.

Thaggikuppe KRISHNAMURTHY.. Praveen

Dr. Praveen T.K.
JSS College of Pharmacy
Ootacamund, Not Applicable, India

He is currently working as professor at Dept. of Pharmacology, JSS College of Pharmacy, Ooty. His research interest include,Computer Aided Drug Discovery, Metabolic disorders-Diabetes Mellitus, Herbal drug research and Preclinical drugdevelopment. Currently, 6 Ph.D., Scholars are working under him guidance and He has guided 25 M. Pharm., students till date.He has published 28 research papers, a Book chapter and successfully filed an Indian patent. He is a reviewer for many Nationaland International Journals.He has received “Summer Research Fellowship-2014” from Science Academies, Govt. of India. Hehas delivered 15 invited Speeches at various National and International Conferences. As a Principal Investigator He is currently carrying out DBT sponsored Major Research Project entitled “Neuroprotection bynanoallosteric potentiators of M1 muscarinic acetylcholine receptors for reversal learning management in Alzheimer’s disease”(Total Grant amount: Rs. 72,19,600/-).