Category: Cellular Technologies
Cancer therapy drugs are usually administered at high doses to have the desired effect on cancer cells; however this can lead to long and short term side effects, and cellular resistance to the drug. Thus, there is a need for targeted cancer therapy drugs to be developed. Quantifying the uptake and effect of targeted drugs has been impossible, traditional methods for testing drug uptake into cells consist of total digestion of the cell population, losing vital information on the mass of drug per cell and the percentage/number of cells containing the drug. Here, we present a new technique, Single Cell ICP-MS (SC-ICP-MS), which is capable of quantifying this. We use the uptake of the chemotherapy drug, cisplatin, into the resistant and non-resistant (A2780 and A2780-CP70) ovarian cancer cell lines. SC-ICP-MS analysis allowed for the quantitation of cisplatin, and other metals, within individual cells yielding information on mass and mass distribution. This technique would be a useful tool in the development of targeted drugs giving fast and in-depth results on the amount of drug and drug distribution in cells that can be linked to biological cellular responses.
Chady Stephan– Product Manager, PKI inc., Woodbridge, ON, Canada
Woodbridge, ON, Canada
Dr. Chady Stephan, Product manager of inorganic solutions at PerkinElmer Health Sciences, holds a Ph.D. in Analytical Chemistry from Université de Montréal, worked as a project manager for QSAR risk as¬sessment services before he joined PerkinElmer as an Inorganic Product Specialist supporting the various elemental analysis platforms. He previously managed the development of various nanotechnology applications that focuses on measurement techniques for nano-object charac¬terization using various analytical platforms. He currently lead a multifunctional team composed of marketing, technical marketing, application scientist and strategist focusing on delivering complete solutions. He is a thought leader in elemental analysis with over 20 peer-reviewed papers and book chapters.