Category: Automation and High-Throughput Technologies

1057-C - How high throughput cell culture automation benefits upstream process development

Tuesday, February 6, 2018
2:00 PM - 3:00 PM

In this poster we will present an overview about our in-house developed fully automated, multiwell plate (MWP) based screening system for suspension cell culture. It is characterized by a fully automated process workflow with integrated analytical instrumentation. It uses shaken 24 well plates as bioreactors which can be run in batch and fed-batch mode with a capacity of more than 600 reactors in parallel. A wide ranging analytical portfolio is available to monitor cell culture processes and a cooperation with internal high throughput analytic groups enables the characterization of product quality. Because of the ideal combination of high throughput cultivation and high throughput analytics the system enables the screening of a wide range of process conditions to optimize product concentration and product quality. The results from the automated multi-well plates are predictive for the bioreactor and the system was established as a routine tool in upstream process development.

The cell culture automation system is completed by an in-house developed and unique Process Control System (PCS). It enables the planning and evaluation of the screening experiments and the fully automated data handling. In this talk we will additionally present our PCS.

In this poster we will additionally present an overview about the benefits for the late stage process development of using cell culture automation in contrast to standard bioreactor systems. For this purpose we will show the experimental results of the development work of several late state projects using the in-house developed cell culture automation system.  We will focus on the increase of the final product concentration by a media screening and on examples to show the capability of the cell culture automation systems to identify product quality levers in just one experiment. All examples will show the potential of cell culture automation as a routine tool in process development.

Carsten Musmann

Roche Diagnostics GmbH
Penzberg, Bayern, Germany

Cell culture automation, Biotechnology, HT Development