Category: Automation and High-Throughput Technologies
Dengue is a mosquito borne infectious disease that has spread rapidly across tropical and sub-tropical regions risking approximately 2.5 billion lives as per World Health Organization(WHO) especially in resource challenged environment. Currently, the specific treatment for dengue is limited and vaccines are in the initial stages of implementation. Hence, early detection would help decrease disease prognosis and mortality rate. The current state-of-art lab techniques take several hours to provide results and require skilled laboratory personnel although accurate and sensitive. Further, these diagnostic tools are expensive and find little scope for application in resource challenged environment. To overcome the existing limitations, there is a need to develop a rapid, affordable and sensitive point of care diagnostic device capable of detecting dengue in its early stages in regions with limited resources.
Amongst existing biosensing techniques, affinity based electrochemical biosensors are promising as they are quick, sensitive and cost effective. In this work, we have developed a novel, low-cost and rapid electrochemical bio sensing platform for early stage NS1 dengue detection. Since non-structural NS1 dengue protein is expressed during the acute phase of infection, it is used as an effective biomarker. To achieve an accurate sensor response a capture probe highly specific to NS1 dengue protein is used. This electrochemical biosensor measures changes in charge transfer resistance and capacitance associated with binding of target antigen to the antibody functionalized electrode surface. We have leveraged the advantages associated with the use of Poly (methyl methacrylate) (PMMA) nanofibers towards developing a sensitive biosensor for early stage NS1 dengue detection in serum using a single drop of sample volume. This low-cost biosensor provides an accurate and rapid response and therefore serves as an ideal point-of-care (POC) diagnostic device in a resource challenged environment for early dengue detection.
Ambalika Tanak– Graduate student, University of Texas At Dallas, Richardson, TX
University of Texas At Dallas
I completed my bachelor's degree in Biomedical engineering from Mumbai University, India in 2013. I completed my Masters in Biomedical engineering from the University of Texas at Dallas (UTD) in 2016. I started my PhD in Biomedical engineering in Fall 2016 in UTD. I am working on making point-of-care diagnostics for infectious diseases which are rapid, cost effective and affordable.