Category: Automation and High-Throughput Technologies
Monoclonal antibodies that bind to specific antigens are fundamental and essential for use as reagents in diagnosis and therapy. Since 2000, the therapeutic market for monoclonal antibody drugs has been increasing exponentially; antibody drugs are expected to lead more than 50% of overall market growth in the future. In contrast to the demand of new antibody drugs is increasing, R&D cost is drastically rising. One of the first obstacles to the development of monoclonal antibody drugs is finding monoclonal antibodies with unique properties from billions of potential candidates.
Antibody phage display technology is a widely used method for screening for specific antibodies against the desired target antigen via enrichment process. With this technology, tremendous variety of antibodies (>1010) are displayed on the surface of the phage. Specific antibodies are selected by enrichment of the bound phage during a series of bio-panning rounds. However, this technology has two major drawbacks. The first drawback is that it is time and cost consuming due to manual work, only a portion of the entire library(<104) can be screened because of labor limitations. The second is that the antibody clones are lost and the enrichment bias of specific clones occurs during the bio-panning process.
Here, we describe a method for identification and isolation of desirable monoclonal antibodies with a high-throughput manner. This method eliminates the unnecessary bio-panning cycles and therefore does not cause biased distribution of specific clones. The entire library can also be screened through the automated system. This approach also provides links between the genotypic and phenotypic information by isolating and sequencing antibody-displayed phage. The system is equipped with microwell-array chip and automated retrieval system with nanosecond pulse laser. The E.coli secreting the antibody-displayed phage are isolated by using microwell. The generated phage are captured for subsequence analysis. After affinity analysis, the bound phage are retrieved by using automated laser system for sequencing. Finally, the sequence information of the desired antibody libraries is obtained. This study potentially provides new ways to screen diverse antibody libraries with a goal for selecting specific monoclonal antibodies against target antigens.
Seohee Chang– Ph.D. course student, Department of Electrical and Computer Engineering, Seoul National University, Gwanak-gu, Seoul-t'ukpyolsi, Republic of Korea
Ph.D. course student
Department of Electrical and Computer Engineering, Seoul National University
Gwanak-gu, Seoul-t'ukpyolsi, Republic of Korea
I am interested in industrialization of lab-based technology especially therapeutic field. And my goal is introducing new technology developed in laboratory to the real-world problem. With the basic knowledge in electrical engineering which i had major in undergraduate, i has been widen knowledge in building a platform and automating system. Recently my work is focused on obstacles of the development of monoclonal antibody drugs which is finding monoclonal antibodies with unique properties from billions of potential candidates.