Category: Micro- and Nanotechnologies

1300-A - A microfluidic system for spatial multilayered cell culture and study on photocytotoxic effect of nanophotosensitizers.

Monday, February 5, 2018
2:00 PM - 3:00 PM

Gynecological and breast cancers are the most common cancers in women. Surgical removal of the tumor and chemotherapy are the most commonly used treatments in present medicine. However, these methods are invasive and not always effective. The promising solution is to use new treatments, e.g. photodynamic therapy (PDT). PDT is a selective and non-invasive method of cancer treatment, which uses light-sensitive compounds known as photosensitizers (PSs). New generation of PSs are nanocarriers, which can improve the effectiveness of treatment and reduce side effects.
The aim of study was to investigated the effect of a photocytotoxic compound, i.e. meso tetraphenylporphyrin nanocarriers (nano-TPP) on ovarian cancer cells (A2780) and non-malignant ovarian fibroblasts (HOF). The research was conducted in a Lab-on-a-Chip microsystem for three-dimensional (3D) multilayer cell culture. Multilayered cell culture is new in vitro spatial cell model, which imitates two elements of cancer construction: non-cancerous stroma and cancer flesh. The microsystem enabled to perform simultaneously two types of analysis: microscopic and fluorescent. The microscopic analysis (e.g. differential staining) is possible thanks to the transparent material used for the microsystem fabrication. Additionally, a microsystem holder allowed the analysis using a commercially available microplate reader. The use of spatial cell cultures in the microsystem allowed to conduct experiments under conditions similar to in vivo environment.
The research was carried out in several stages. Non-malignant fibroblast cells were introduced into the microsystem and next day, when these cells got adhesion, the cancer cells were introduced. On the third day, various concentrations of nano-TPP solutions (0÷30 μM) were introduced into the microsystem and incubated for 24h. After incubation with nano-TTP, the cells were irradiated with light (λ=625nm, 40mW/cm2) for 10 min (PDT procedure). The cell viability was daily monitored using alamarBlue® assay and microscopic observation. The results of cytotoxicity studies show, that the lowest non-cytotoxic nano-TPP concentration is 5 μM. This concentration of compound was used to perform the PDT procedure and the significant photocytotoxic effect was observed in 3D cell culture. The results of fluorescent analysis show, that 24h after PDT the cells’ viability decrease by 60% of control.
The results show, that the developed microsystem could be appropriate microtool to create new 3D cancer models and evaluate the effectiveness of anticancer therapy. In the future the microsystem can be used for examination new nanocarriers with PSs and other anticancer drugs.

Magdalena Bulka

PhD Student
Warsaw University of Technology
Warsaw, Mazowieckie, Poland

I was born in Poland in 1991. I received my Eng. In biotechnology from the Faculty of Chemistry, Warsaw University of Technology (Poland) in 2014. I finished my Master thesis in Department of Microbioanalytics. The subject matter of my research was study on photocytotoxic properties of selected photosensitizers using the microsystems called Lab-on-a-chip. Currently I am a PhD student in the Department of Microbioanalitycs and I am a member of the Chemical Sensor Research Group. My current research interests are designing and fabrication of microfluidic Lab-on-a-chip systems dedicated for anticancer therapies. I am currently working on new spatial (3D) cell models, cultured in microfluidic system, for drug screening and conduct automated analyzes.