Poster Topical Area: Aging and Chronic Disease
Poster Board Number: 31
Background: Ulcerative colitis (UC) causes recurring intestinal mucosal injury and sustained inflammation, increasing the likelihood of colorectal cancer (CRC)-development. Dietary red-raspberry (RB) is a rich source of polyphenols known to have anti-inflammatory and anti-oxidative activities, however, the role of RB on CRC-prevention in chronic colitis has not been examined.
Objective: The objective of current study was to examine the effect of dietary RB-supplementation on inflammation, epithelium repair and oncogenic signaling in DSS-induced chronic colitis in mice. We hypothesized that RB facilitates epithelium repair and reduces CRC-risk due to its anti-inflammatory property.
Methods: Six-week-old-male (C57BL/6J) mice were fed a control or RB (5% of dry feed weight, n=12/group) diet for 10-weeks. Starting the 4th-week, mice were subjected to 2-repeated cycles of 1% DSS (7-day-DSS-treatment plus 14-day-recovery) and were monitored daily for disease activity index (DAI). Colonic tissues were collected at the end of study for histochemical/immunohistochemical and biochemical analysis.
Results: RB-supplementation reduced the DAI and histological damage (P £ 0.01), expression of inflammatory mediators (P £ 0.01), infiltration of CD4 T cells (P £ 0.05), and α4β7 integrin and related adhesion molecules (P £ 0.01). Furthermore, RB-supplementation facilitated epithelium repair evident by enhanced goblet cell density, expression of transcription factors including Kruppel-like factor-4 (Klf-4) and Hairy and enhancer of split 1 (Hes-1), terminal differentiation markers, Muc-2 and intestinal alkaline phosphatase (P £ 0.01). Conversely, proliferating cell nuclear antigen (PCNA, P £ 0.01), as well as β-catenin and signal transducer and activator of transcription3 (STAT3) signaling were reduced (P £ 0.05) by RB-supplementation. In addition, RB-supplementation enhanced p53 stability and reduced oncogenic gene expression.
Conclusion: RB-supplementation reduced DAI and risk of CRC-development during recurring colitis in mice, suggesting that RB is a good dietary supplement for patients with UC and related gut inflammatory diseases.