Poster Topical Area: Vitamins and Minerals
Location: Hall D
Poster Board Number: 539
Background: Selenium (Se), an essential nutrient element, has significantly protective effects against testis damage induced by some heavy metals. Cadmium (Cd) is a ubiquitous environmental pollutant that associated with male reproductive dysfunction. However, whether nutrition intervention of Se could against Cd-induced male reproductive dysfunction on chicken is unknown.
Object: We attempted to characterize the relationship between different dietary Se, the selenoprotein, and the reproductive function in Cd-exposed male chicken. And filtrate the best intervention from different Se sources on Cd toxicity.
Method: 1-day-old male chickens were randomly allocated to five groups and oral administrated with different Se sources (1 mg/kg Nano-Se, 3 mg/kg Yeast-Se or 3 mg/kg Na2SeO3) with or without CdCl2 (140 mg/kg) for 90 days, respectively. Histological and ultrastructural changes, body weight gain, testis coefficient, oxidative stress biomarkers, serum sex hormones and biochemical indexes levels were determined. The expression of selenoproteome of 24 selenoproteins were detected by qRT-PCR array.
Results: The results revealed that Cd had no effect on mortality but depressed feed intake and growth. Cd influenced the biochemical parameters and redox balance in testis. The arrested development of testis and the downregulated concentrations of LH, PRL and T were observed in Cd-exposed chicken. Nutrition intervention of Se significantly ameliorated Cd-induced oxidative damage and endocrine disorder. Notably, the expression of selenoproteome has a hierarch while changing Se status, and 24 selenoproteins levels were not identical by regulation of different Se dietary.
Conclusion: Se supplementation had chemopreventive potential against Cd-induced testicular toxicity. Furthermore, yeast-Se and nano-Se intervention had more obvious advantages relative to inorganic Se by regulation of selenoprotemic transcriptions. We hypothesize that hierarchy of regulated selenoprotein expression makes an important role of Se metabolism and transport in Cd-exposed testis.
*Corresponding author: Jin-Long Li. Address: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, P. R. China. Tel: +86 451 55190407; fax: +86 451 55190407. E-mail address: Jinlongli@neau.edu.cn
Funding Source: Support or Funding Information: National Natural Science Foundation of China (No. 31572586), Excellent Youth Foundation of Heilongjiang Province of China (No. JC2017005), Academic Backbone Project of Northeast Agricultural University (No. 15XG16) and China Agriculture Research System (No. CARS-35).
College of Veterinary Medicine, Northeast Agricultural University
Harbin, Heilongjiang, China (People's Republic)