Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 334
Objective: The present study aimed to investigate the potential mechanism of M. citrifolia L. (MC) fruit extract on glycemic control and anti-diabetic retinopathy using proton nuclear magnetic resonance (1H-NMR)-based metabolomics approach.
Method: Forty male Sprague-Dawley rats were divided into 5 groups [Control; Diabetic (DB); DB treated with 200mg/kg ethanolic MC extract (DB+MC200); DB treated with 400mg/kg ethanolic MC extract (DB+MC400) and DB treated with 5mg/kg Gliclazide (DB+DG)] and were subjected to 8 weeks of treatment. Glycemic responses were monitored on weekly basis, sampled from the tail vein. Retinal examination was conducted through a dilated pupil using direct ophthalmoscope and portable slit lamp biomicroscopy. Serum obtained upon sacrificed were analyzed for glucose, lipid profiles and subjected to 1HNMR-based metabolomics profiling using untargeted approach. Unsupervised principal component analysis (PCA) was used to discriminate serum metabolomics profile of different rat groups and explore the change of associated biochemical pathway. Relative concentration of the metabolites responsible for the different profile was quantified using Chenomx NMR Suite.
Results: The results obtained indicated that MC400 and MC200 extracts were equally as effective as gliclazide in term of glycemic control. At the end of 8 weeks, 86% (n=6) of animal in DB group had moderate and severe diabetic cataract, as opposed to 50% (n=4) in DB+MC200, 25% in DB+MC400 (n=2) and 50% (n=4) in DB+DG group had only mild diabetic cataract. None of the animal in control group developed cataract. PCA score plots showed clustering of the rat in the first and second principal components, with separation between DB, DB+treatment groups and control. DB+MC400 rats had metabolic perturbation closest to control rats, suggesting its effectiveness as anti-diabetic agent. Quantitative analysis showed that DB+MC200, DB+MC400 and DB+DG had varying significant reduction of glucose, 3-hydroxybutyrate, acetone, acetoacetate whilst elevated level of acetate, pyruvate, succinate and alanine when compared to DB.
Conclusion: MC fruit extract administered at 400mg/kg are effective as hypoglycemic agent and delayed diabetic retinopathy in diabetic induced rats, thus warrant further investigation in clinical setting.
This research has been funded by NKEA Research Grant Scheme (NRGS) under the Ministry of Agriculture Malaysia.
Zulfitri Azuan Mat Daud
Universiti Putra Malaysia
Mantin, Negeri Sembilan, Malaysia